Scary science. Effect of DDT and other toxins on fish sleep and behavioral activity via computer vision behavior mathematical decomposition. I do not like this. Behavior is not mathematics.
Electrophysiology high throughput device for screening drungs in cell cultures with cardiomyocytes. This kind of technology enables high throughput screening of small molecules against molecular targests quickly with miniaturized hybrid electronic-biological technology. The cells in the monolayer beat together in unison. This is very interesting!
Online analysis, this enables you to monitor the device via the web.
You can evaluate the stability of your monolayer this way.
Compound editor. Gives you the ability to create a 96 well assembly of compounds.
Seeding density determines the emergence of collective properties in the network of cells.
I wonder, what have people done with CDK-pathways with screens for molecules with technology like this?
Video games, I wonder if there are some movies on the collective beating of cardiomyocytes?
Are there some videos on YouTube with this kind of “emergent” phenomenon?
Wells are organized by compound names.
Scroll through wells and see individual compound effects or mean CRC of all wells.
Detection and quantification of secondary beats
Base impedance the steady component of the measured impedance value
Beat rate regularity index (BRRI)
What is the best channel?
White light stimulate one point in the monolayer and study the propgation of waves of excitation in the monolayer
When we see the biphasic peaks, her blockers…
IKR, herk, potassium channel
Monolayers of stem cells
3 Hz = ~180 bpm
Artificially imposed beat rate
How does this device artificially impose a beat rate?
Cell adhesion and spreading
A question that comes to mind is the issue of pleotropy. Has anyone done a high throughput screen to look at a systems level analysis of cross reactivity of cardioacative drugs on genes?
The issue that I thought of was that of the off target effects of anti-psychotics like Seroquel.
What has been done with high throughput screening in cardiomyocytes?
Cite Zebrafish study in this blog entry.
The work of the company in Boston that is looking at compounds in zebrafish in a high throughput manner.
I feel this kind of work is unethical and against the Qur’an and the spiritual energy of God and Gaia. Can we find a way to listen to earth and create peaceful harmonious energy in science like this? Yes! 🙂
Agriculture and greenness in spiritual harmony:
— Kaya Erbil (@WilliamKaya) December 8, 2015